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University of Wisconsin–Madison

Imaging the Third Dimension at the Nanometer Resolution

This project funds acquisition of a 3View serial block face scanning electron microscope (SBFSEM) that will provide UW–Madison researchers the opportunity for large scale 3D reconstructions of cellular structures and connectivity measures at the finest resolution currently achievable and at an accuracy and speed not attainable by other imaging methods.

Many research labs and centers across the world have turned to serial block face SEM over the past decade to answer a vast number of questions about the structure/function of the mouse and primate nervous system, the zebrafish brain, fly brain, cornea, human hepatoma cells, carotid artery elastin, zebrafish heart, epidermal melanocytes, collagen fibrils, cardiac myocytes, chromatic architecture of hepatocytes and kidney ultrastructure, olfactory bulb, trophozoite, plant tubular networks and brain mitochondria. Given its vast applications across biology; almost any researcher interested in cell biology and cellular organization will have applications for the SBFSEM.

Principal Investigator:

  • Mrinalini Hoon
    Assistant Professor of Ophthalmology and Visual Sciences

Co-Principal Investigators:

  • Anjon Audhya
    Associate Professor of Biomolecular Chemistry
  • Kevin Eliceiri
    Principal Investigator and Director of the Morgridge Institute of Research Laboratory for Optical and Computational Instrumentation
  • Marisa Otegui
    Professor of Botany
  • Raunak Sinha
    Assistant Professor of Neuroscience
  • Phillip Smith
    Professor of Neuroscience


  • Sebastian Bednarek
    Professor of Biochemistry
  • Edwin Chapman
    Professor of Neuroscience
  • Chiara Cirellia
    Professor of Psychiatry
  • LaTasha Crawford
    Assistant Professor of Pathobiological Sciences
  • David Gamm
    Associate Professor of Ophthalmology and Visual Sciences
  • Angela Gibson
    Assistant Professor of Surgery
  • Xin Huang
    Associate Professor of Neuroscience
  • Kip Ludwig
    Associate Professor of Biomedical Engineering
  • Gillian McLellan
    Associate Professor of Ophthalmology and Visual Sciences
  • Darcie Moore
    Assistant Professor of Neuroscience
  • William Murphy
    Professor of Biomedical Engineering
  • Robert Nickells
    Professor of Ophthalmology and Visual Sciences
  • Bikash Pattnaik
    Associate Professor of Pediatrics
  • Francisco Pelegri
    Professor of Genetics
  • Samuel O. Poore
    Assistant Professor of Surgery
  • Jeremy D. Rogers
    Assistant Professor of Biomedical Engineering
  • Ari Rosenberg
    Assistant Professor of Neuroscience
  • Yuri Saalmann
    Assistant Professor of Psychology
  • Jayshree Samanta
    Assistant Professor of Comparative Biosciences
  • Aussie Suzuki
    Assistant Professor of Oncology
  • John Svaren
    Professor of Comparative Biosciences
  • Jill C. Wildonger
    Assistant Professor of Biochemistry
  • Justin Williams
    Professor of Biomedical Engineering
  • Elizabeth Wright
    Professor of Biochemistry
  • Mostafa Zamanian
    Assistant Professor of Pathobiological Sciences
  • Xinyu Zhao
    Professor of Neuroscience